Title
Osteoclast Impairment in Chronic Periodontitis by Regenerative Materials.
Platelet Rich Fibrin/Biphasic Calcium Phosphate Impairs Osteoclasts Differentiation and Promotes Apoptosis by the Intrinsic Mitochondrial Pathway in Chronic Periodontitis
Phase
N/ALead Sponsor
Meenakshi Ammal Dental College and HospitalStudy Type
InterventionalStatus
Completed No Results PostedIndication/Condition
Periodontal Disease Periodontal Bone Loss Osteoclastic Bone LossIntervention/Treatment
other : PRFStudy Participants
50The present study is an effort to investigate the hypothesis that Platelet Rich Fibrin/Biphasic Calcium Phosphate may play a role in inhibiting osteoclasts differentiation and in bone loss.
Study design
Fifty-five individuals from a suburban population were recruited. Out of them, 5 individuals refused to participate in the study. Finally, 50 participants were selected and divided into two groups, control group [Healthy individuals]: 25 Males/Females, aged 25 to 35 years and test group [Chronic periodontitis patients]: 25 Males/Females aged 30 to 45 years.
Demographic variables and periodontal parameters were measured in both the groups.The investigators explored the effect of Platelet rich fibrin (PRF)/Biphasic calcium phosphate (BCP) on differentiation and survival of osteoclasts obtained from peripheral blood of Chronic Periodontitis patients.
mononuclear cells from the peripheral blood of chronic periodontitis patients were intervened with PRF, BCP and PRF/BCP combination
Mononuclear cells isolated from the peripheral blood of 25 chronic periodontitis patients were interveened with 1ml/well preparation of Platelet rich fibrin(PRF), Biphasic calcium phosphate (BCP) and PRF and BCP combination in-vitro.
Mononuclear cells isolated from the peripheral blood of healthy individuals in-vitro.
Inclusion criteria: (Control group) Subjects with age group between 30 to 45 years. Non-smokers. No previous history of periodontal treatment. Inclusion Criteria: (Test group) Subjects with age group between 30 to 45 years. Presence of atleast 20 teeth More than 30% of the sites with clinical attachment loss. Non-smokers. No previous history of periodontal treatment. Exclusion Criteria: ( both the groups) Individuals with systemic disease Subjects under antibiotics or anti-inflammatory drugs within the previous 3 months. Pregnant and lactating women. Allergic to antibiotics.