Trial | NCT01005342  Report issue

Title

Influence of Dietary Fiber-rich Meals on Gene Expression and Postprandial Glucose and Lipid Response
The Influence of Dietary Fibre-rich Meals on Gene Expression in Leukocytes and Postprandial Glucose and Lipid Response in Healthy Subjects

  • Phase

    N/A

  • Study Type

    Interventional

  • Status

    Completed No Results Posted

  • Intervention/Treatment

    Oat bran ...

  • Study Participants

    18
The aim of this study is to

Measure the effect on gene expression in leukocytes from a meal rich in oat bran
Investigate the postprandial glucose, insulin and triglyceride responses after intake of meals containing fiber from different sources (oat, rye and sugar beet fiber) or a meal containing a mixture of these three fibers
Dietary fiber has long been known to give beneficial health effects. Yet, the understanding of how fiber-rich meals regulate molecular events at a gene level is limited. Also, few studies have compared the effects of different fiber sources on postprandial responses and hardly any study the effects of fiber mixtures in the same meal, even though this is more similar to regular eating habits.

Healthy subjects will come to the study center after an overnight fast, to ingest breakfasts randomly enriched with different fiber. The meals contains either spray-dried oat drink, rye bran, sugar beet fiber a mixture of these three fibers, oat bran or no added fiber (control). All meals are adjusted to contain the same total amount of available carbohydrates and fat. Blood leukocytes for gene expression profiling were taken before and 2 h after consumption while blood samples for analysis of postprandial glucose, insulin and triglyceride levels were taken every 30 min during 3 h.

NuGO Affymetrix Human Genechip NuGO_Hs1a520180 are used for the microarray analysis and analysis is performed with linear mixed models and enrichment analysis to identify functional gene sets that responded to the specific oat bran effect.
Study Started
May 31
2007
Primary Completion
Jun 30
2007
Study Completion
Jun 30
2007
Last Update
Oct 30
2009
Estimate

Other Oat bran

82 g oat bran was added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (12.6 g total fiber).

  • Other names: Oats, Beta-glucan

Other Spray-dried oat drink

62 g spray-dried oat drink was added to 250 ml blackcurrant beverage with pulp to give 2.7 g soluble fiber (3.3 g total fiber).

  • Other names: Oats, Oat milk, Beta-glucan

Other Rye bran

31 g rye bran was added to 250 ml blackcurrant beverage with pulp to give 1.7 g soluble fiber (12 g total fiber).

  • Other names: Rye, Arabinoxylan

Other Sugar beet fiber

19 g sugar beet fiber was added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (12 g total fiber).

  • Other names: Sugar beet, Pectin

Other Mixture of fiber

38 g spray-dried oat drink, 30 g rye bran and 6 g sugar beet fiber were added to 250 ml blackcurrant beverage with pulp to give 5 g soluble fiber (18 g total fiber).

Other Control

No fiber was added to control meal (250 g black-currant beverage)

Mixture of fiber Experimental

Single intake of a mixture of spray-dried oat drink, rye bran and sugar beet fiber

Sugar beet fiber Experimental

Single intake of sugar beet fiber

Rye bran Experimental

Single intake of rye bran

Oat bran Experimental

Single intake of oat bran

Spray-dried oat drink Experimental

Single intake of spray-dried oat drink

Control Placebo Comparator

Single intake of a meal with no added fiber

Criteria 

Inclusion Criteria:

BMI 18-30

Exclusion Criteria:

pregnancy
breastfeeding
diabetes mellitus
hepatitis B
blood lipid lowering pharmaceuticals
intolerance or allergy to cereals or sugar beet fiber
No Results Posted