Title
Study of Evaluation Indexes for Antibody Production in Sera After Immunization
A Study of Evaluation Indexes for Antibody Production in Sera After Immunization
Phase
Phase 4Lead Sponsor
Ewha Womans UniversityStudy Type
InterventionalStatus
Completed No Results PostedIndication/Condition
AgedIntervention/Treatment
23-valent pneumococcal polysaccharide vaccine ...Study Participants
70The aim of this study is to evaluate analytical methods such as ELISA and OPKA that measure the activity of vaccine-induced pneumococcal antibodies. After validation of ELISA and OPKA, pneumococcal antibodies were measured by ELISA and OPKA in paired samples before and after immunization (pneumococcal PS vaccine) in children, adults and elderly.
To evaluate the immune response to the pneumococcal polysaccharide vaccine in the elderly, samples from children, young adults and elderly were obtained before and one month after vaccination. The quantitative and qualitative response to the vaccine were measured by the ELISA and opsonophagocytic killing assay for eight vaccine type serotypes (4, 6B, 9V, 14, 18C, 19A, 19F, 23F) and one vaccine-related serotype (6A).
single intramuscular injection of 0.5 ml Pneumo23® (Sanofi-Aventis, Lyon France)
The immunogenic response to the pneumococcal polysaccharide vaccine was compared between children, adults and elderly.
Inclusion Criteria: elderly subjects over the age 65 years who had not received pneumococcal vaccination adult subjects under the age of 45 years (healthy volunteers with no previous history of pneumococcal vaccination) children subjects over the age of 2 years with no previous history of pneumococcal vaccination Exclusion Criteria: immunocompromised, asplenia, cancer, liver or renal failure, and history of hypersensitivity to vaccine
