Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E] » Investigative Techniques [E05] » Chemistry Techniques, Analytical » Electrophoresis » Isotachophoresis
Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E] » Investigative Techniques [E05] » Electrochemical Techniques » Electrophoresis » Isotachophoresis
Description
A nonlinear electrophoretic technique used to separate a variety of ionic compounds, ranging from small metal ions to large molecules like proteins. Unlike "linear" zone electrophoresis in which separating solute bands continually spread by diffusion or dispersion, isotachophoresis forms self-sharpening, adjacent zones of substantially pure solute whose concentrations often exceed several mgs/ml. In isotachophoresis a multianalyte sample is introduced between the leading electrolyte and the terminating electrolyte where the sample ions have lower electrophoretic mobilities than the leading ion but larger than the terminating ion. (From "Isotachophoresis" on the AES Web Site [Internet]. Madison, WI: The American Electrophoresis Society; c2000-2008 [cited 2009 Aug 20]. Available from http://www.aesociety.org/areas/isotachophoresis.php) MeSH
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