Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E] » Investigative Techniques [E05] » Genetic Techniques » Chromatin Immunoprecipitation
Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E] » Investigative Techniques [E05] » Immunologic Techniques » Immunoprecipitation » Chromatin Immunoprecipitation
Description
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing. MeSH
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