Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E] » Investigative Techniques [E05] » Genetic Techniques » Nucleic Acid Hybridization
Phenomena and Processes [G] » Chemical Phenomena [G02] » Biochemical Phenomena » Nucleic Acid Hybridization
Description
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) MeSH
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