Flow Cytometry D005434

Related MeSH Hierarchy (7)

Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E] » Diagnosis [E01] » Diagnostic Techniques and Procedures » Clinical Laboratory Techniques » Cytological Techniques » Cell Separation » Flow Cytometry

Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E] » Diagnosis [E01] » Diagnostic Techniques and Procedures » Clinical Laboratory Techniques » Cytological Techniques » Cytophotometry » Flow Cytometry

Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E] » Investigative Techniques [E05] » Chemistry Techniques, Analytical » Photometry » Luminescent Measurements » Fluorometry » Cytophotometry » Flow Cytometry

Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E] » Investigative Techniques [E05] » Clinical Laboratory Techniques » Cytological Techniques » Cell Separation » Flow Cytometry

Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E] » Investigative Techniques [E05] » Clinical Laboratory Techniques » Cytological Techniques » Cytophotometry » Flow Cytometry

Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E] » Investigative Techniques [E05] » Cytological Techniques » Cell Separation » Flow Cytometry

Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E] » Investigative Techniques [E05] » Cytological Techniques » Cytophotometry » Flow Cytometry

Description

Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.   MeSH

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